Figure 2.

Detection of galactosylated glycoproteins by using R. communis RCA₁₂₀ affinity chromatography. Eluted fractions were subjected to SDS/PAGE, and the gel was visualized by silver staining (A) or blotted onto a nitrocellulose membrane and lectin (RCA₁₂₀) stained (B). Lanes 1 and 2, proteins from wild-type BY2 (WT); 3 and 4, proteins from transformed GT6. (C) Blots probed with a xylose-specific antiserum for plant complex glycans. Lanes 1 and 2, total protein extracts from BY2 and GT6; 3, glycoproteins from GT6 after RCA₁₂₀ affinity chromatography. Molecular mass markers are in kDa.