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. 1999 Apr 13;96(8):4698–4703. doi: 10.1073/pnas.96.8.4698

Figure 2.

Figure 2

Radiochromatograms showing separation by HPLC of 14C-labeled GA substrates and products after incubation with recombinant PcGA2ox1. GA15cl and GA15ol refer, respectively, to the closed- and open-lactone forms of GA15. Products were identified as methyl esters trimethylsilyl ethers by GC/MS by comparison of their mass spectra with published spectra (27): putative [17-14C]2β-hydroxyGA15 mass/charge (% relative abundance), 434(72), 419(12), 402(21), 376(16), 312(33), 284(100), 256(31), 239(94), 195(41), 157(91); [17-14C]GA51, 420(3), 388(19), 330(19), 298(19), 286(80), 284(28), 243(27), 229(39), 227(100), 226(58), 143(33); [17-14C]GA51-catabolite (as enol), 432(100), 417(5), 373(18), 357(13), 313(49), 283(18), 269(20); [17-14C]GA29, 508(100), 493(8), 479(6), 449(8), 391(11), 377(8), 305(29), 237(8), 209(37); [17-14C]GA34, 508(100), 418(9), 374(6), 358(5), 313(10), 290(16), 263(11), 231(22), 225(21), 217(32), 203(21), 147(31); [17-14C]GA34-catabolite (as ketone), 448(39), 389(19), 373(52), 329(100), 313(66), 299(22), 260(30), 239(51), 201(68) (from a different set of incubations); [17-14C]GA8, 596(100), 594 (78), 581(7), 579(5), 537(8), 450(20), 448(17), 381(9), 379(13), 377(13), 313(8), 283(11), 240(15), 238(15), 209(30), 207(28).