Alignment of deduced amino acid sequences of GA 2-oxidases and other dioxygenases of GA biosynthesis. Dark shading indicates amino acid residues that are conserved in the GA 2-oxidases. ∗, Amino acid residues presumed to bind Fe at the active site. M7–3, putative dioxygenase of undefined function from M. macrocarpus (accession no. Y09113); AtGA20ox1, GA 20-oxidase from Arabidopsis encoded by the GA5 gene (accession no. X83379); AtGA3ox1, GA 3β-hydroxylase from Arabidopsis encoded by the GA4 gene (accession no. L37126); CmGA23ox1, GA 2β, 3β-dihydroxylase from pumpkin (accession no. U63650).