Skip to main content
. 1999 Apr 13;96(8):4698–4703. doi: 10.1073/pnas.96.8.4698

Figure 4.

Figure 4

Expression of GA 2-oxidase genes in Arabidopsis. (A) Tissue distribution of transcript in ecotype Columbia. Northern blots of poly(A)+ RNA (5 μg) from roots (Rt), leaves (Lf), lower stems (LS), upper stems (US), flowers (Fl), and siliques (Si) were hybridized to 32P-labeled cDNA for AtGA2ox1, AtGA2ox2, and actin (Act-2 gene, base pairs 1447–1832) (28). (B) Effect of GA on transcript abundance. Poly(A)+ RNA (5 μg) from immature flower buds of the GA-deficient ga1–2 mutant of Arabidopsis, either untreated (−GA3) or treated 24 h previously with 10 μM GA3 (+GA3), was hybridized to 32P-labeled cDNA for AtGA2ox1, AtGA2ox2, AtGA20ox2, AtGA3ox1, and Act-2.