Figure 4.

Interaction between AtCBL1 and rat CNA protein in the yeast two-hybrid system. Growth of yeast transformants on SC −Trp −Leu medium (A and B), SC −Trp −Leu −His containing 25 mM 3-aminotriazole (C and D), and the colony color of the transformants determined by the filter lift assay (E and F) are shown. Plates containing histidine, but lacking tryptophan and leucine, select for the presence of the two plasmids containing the Gal4 DNA-binding and the Gal4 activation domain. Plates lacking tryptophan, leucine, and histidine and supplemented with 25 mM 3-aminotriazole select for a positive interaction between the fusion proteins. The array of the yeasts containing the different constructs is indicated in the scheme at the bottom. The symbols for the constructs were described in the text.