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Detection of VPg in protein extracts from CYDV-infected oat plants by immunoblotting. The total protein from healthy (lane 1) or CYDV-infected (lane 2) oat plants was subjected to SDS-PAGE and then blotted onto a membrane and probed with a CYDV bVPg antiserum. The migration positions and sizes of two proteins with known molecular sizes (Sigma; ColorBurst) which were coelectrophoresed in an adjacent lane are indicated by arrows.
