FIG. 7.

Effects of mutations in the predicted 3′-terminal stem-loop structure on the template activities of CYDV plus-strand RNAs. RdRp reactions were carried out and the products analyzed as described in the legend to Fig. 5. Lane 1 contains wild-type (WT) RNA, and lanes 2 to 6 contain mutant RNAs K, J, M, L, and N, respectively, as described in the text. An 8% gel was used for lanes 1 to 5 and a 12% gel for lane 6. The products and their sizes are indicated by arrows.