FIG. 8.

Initiation of CYDV minus-strand RNA synthesis by addition of nucleotides to bVPg. The reactions were carried out using BAL 31 nuclease-treated CYDV RdRp, and the reaction products were analyzed by SDS-PAGE (10% gels) and autoradiography. The template, when present, was an RNA corresponding to the 323 3′-terminal nucleotides of wild-type CYDV RNA plus strand (WT) or mutant H CYDV RNA (H). Lanes 1 to 6 contained 10 μM [α-³²P]ATP, and lanes 7 to 9 contained 250 μM [α-³²P]ATP. Lanes 5 and 6 contained 10 μM CTP, and lanes 8 and 9 contained 250 μM CTP. The fastest-migrating products are indicated by an arrow.