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KSHV-infected TIME cells stabilize more HIF alpha subunits when exposed to the hypoxia mimic. Western blot analysis of mock (lanes 1 and 3)- and KSHV-infected (lanes 2 and 4) TIME cell nuclear extracts at 48 h postinfection in the absence (−) (lanes 1 and 2) or presence (+) (lanes 3 and 4) of an HIF-stabilizing hypoxia mimic (150 μM DFO) for the last 8 h. The extracts were probed with antibodies against HIF1α, HIF2α, HIF1β, and the load control, TFIIβ.
