FIG. 3.

KSHV-infected TIME cells stabilize more HIF alpha subunits in the presence (+) of a proteosome inhibitor. Western blot analysis of mock (lanes 1 to 3)- and KSHV-infected nuclear extracts (lanes 4 to 6) at 24 h postinfection. The extracts were treated with a proteosome inhibitor (20 μM MG132) for either 1 h (lanes 2 and 5) or 2 h (lane 3 and 6) to stabilize HIF and probed with antibodies against HIF1α, HIF2α, HIF1β, and the load control, TFIIβ. −, absence of KSHV infection; +, presence of KSHV infection.