FIG. 3.

NFκB is activated during HCMV infection. (A) HFF cells were mock transduced or transduced with adenoviruses expressing IE86 or GFP and treated with 50 ng/ml TNF-α. Cell lysates were prepared 30 min posttreatment and assayed for phosphorylated IκBα, total IκBα, and tubulin by Western blot analysis. HFF cells (B) or HFF cells transduced with adenovirus expressing IE86 (C) were seeded onto coverslips and either mock infected or infected with WT-HCMV or UV-HCMV. Cells were fixed 3 h postinfection and assayed for NFκB (p50) and IE86 localization by an immunofluorescence assay. Nuclei were stained with Hoechst.