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MAbs that neutralize clinical isolates of HEV neutralize HEV produced in vitro. The number of infectious virions remaining after overnight incubation with PBS or with a panel of MAbs specific for ORF2 protein was quantified on 10-3 cells. Pairs of columns represent duplicate samples. MAbs mapping to antigenic site 2 were the only MAbs to neutralize clinical isolates (17). The epitope for EBL1 bridged antigenic sites 1 and 2.
