FIG. 6.

Sucrose velocity sedimentation gradients of lysates from cells transfected with the wild-type, ORF3-null, or ORF2-null viral genomes or of a rhesus stool suspension containing wild-type HEV. Note the differences in the abscissa scales. All samples except the sample in panel A were treated with RNase, but the sample in panel A received less RNase than the samples in panels C to F. Samples in panels A and B were spiked with HAV. Samples in panels C, D, and E were prepared in parallel starting at the transfection step, and the sample in panel F was included at the RNase step. The numbers of viral RNA genomes loaded onto gradients in panels C, D, and E were 3.1 × 10⁶, 3.8 × 10⁶, and 2.3 × 10⁶, respectively.