TABLE 1.
Comparison of Huh-7 subclones for transfectability and infectibility by HEV
| Subclone | Transfection efficiencya (GFP intensity) | No. of cells infectedb |
|---|---|---|
| 10-3 | 4+ (3+) | 273 |
| 10-7 | 1+ (2+) | 5 |
| 10-8 | 1+ (2+) | 17 |
| 10-9 | 2+ (2+) | 48 |
| 10-10 | 4+ (4+) | 42 |
| 10-11 | 1+ (2+) | 49 |
| 13-1 | 3+ (3+) | 9 |
a
Cells were transfected with an HEV replicon encoding GFP, and the proportion of cells producing GFP and the GFP fluorescence intensity were estimated on a scale of 0 to 4.
b
Five days after cultures were incubated with a human stool suspension containing HEV, the cells were stained with anti-ORF2 protein for immunofluorescence microscopy, and the number of cells containing ORF2 capsid protein was tallied.