TABLE 2.
Infection assay for wild-type HEV and mutants
| Inoculuma | No. of positive cellsb |
|---|---|
| Wild-type virus | 34, 32 |
| ORF2-null mutant virus | 0, 0 |
| HEV replicon encoding GFP | 0, 0 |
a
Inocula were prepared by transfecting 10-3 cells in parallel with the indicated genomes and harvesting the cells on day 9.
b
Cultures were inoculated with identical amounts of cell lysate, and the number of cells per well exhibiting green fluorescence due to immunofluorescence staining of ORF2 (wild-type virus) or ORF3 protein (ORF2-null mutant) with Alexa Fluor 488 or due to production of GFP (replicon) were tallied. The values for duplicate samples are given.