FIG. 7.

A238L inhibits NF-κB-p65 and p300 transactivation. Raw-pcDNA (gray bars) and Raw-A238L (black bars) cells were cotransfected with GAL4-luc (150 ng DNA/10⁶ cells) and GAL4-p65 (A) or GAL4-p300 (B), at a final concentration of 50 ng DNA/10⁶ cells, and cultured with 1 μg/ml of LPS plus 200 U/ml of IFN-γ. At the indicated poststimulation times, whole-cell extracts were prepared and luciferase activity was assayed. Extracts were normalized to Renilla luciferase activity as described in Materials and Methods. RLU per μg of protein from triplicate transfections (means ± SD) are shown.