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. 2006 Aug 23;80(21):10534–10541. doi: 10.1128/JVI.01172-06

FIG. 2.

FIG. 2.

Determination of UL11 copy number. Equal amounts of radiolabeled, gradient-purified HSV-1 virions were separated by SDS-PAGE on 8% and 15% polyacrylamide gels, and proteins were visualized by autoradiography. VP5 was identified in these preparations based on its unique size, whereas VP16 and UL11 were identified by immunoprecipitating the respective proteins from radiolabeled, infected-cell lysate using specific antibodies: anti-VP16 (αVP16) and anti-UL11 (αUL11). All three proteins were quantitated using PhosphorImager analysis and normalized for the number of cysteines in each protein.