FIG. 2.

LMP1 TM1-2ΔC induction of TM3-6-mediated NF-κB activation is TM1F₃₈WLY₄₁ dependent. Full-length FLAG-tagged LMP1 (FTM1-6) or FTM3-6 and increasing amounts of HTM1-2ΔC or its mutant HTM1-2ΔC A₃₈ALA₄₁ were expressed in HEK293 cells. The HEK293 cells were also transfected with a plasmid with five Igκ NF-κB sites preceding a minimal promoter and luciferase reporter and a pGK-β-gal reporter control plasmid. Cells were lysed after 24 h and assayed for luciferase, β-galactosidase, and LMP1 C terminus expression. Luciferase values were normalized for β-galactosidase activity. FTM1-6-induced luciferase activity was set to 100% as an internal marker for each experiment. The presented data are representative of three independent transfections done in duplicate. The LMP1 C terminus (CT) was identified with S12 monoclonal antibody (41).