FIG. 4.

LMP1 TM1-2ΔC can weakly induce TM5-6-mediated NF-κB activation, and TM1 A₃₈ALA₄₁ has little effect. NF-κB activation was measured after HTM1-2ΔC or its mutant HTM1-2ΔC A₃₈ALA₄₁ was expressed alone or with FTM3-6 or FTM5-6 in HEK293 cells. NF-κB activation was measured by cotransfection with a plasmid with five Igκ NF-κB sites preceding a minimal promoter and luciferase reporter and a pGK-β-gal reporter control plasmid and analyzed as described in the legend for Fig. 2. Two independent experiments, one where expression of FTM5-6 is low in comparison to FTM3-6 (A) and one where expression of FTM5-6 is high (B), are shown.