FIG. 6.

LMP1 TM1-2ΔC interaction with TM3-6 is partially dependent on TM3W₉₈. GST alone, GST-tagged TM1-2ΔC (GTM1-2ΔC), or its mutant GTM1-2ΔC A₃₈ALA₄₁ was expressed in HEK293 cells with FTM3-6, FTM3-6 W₉₈A, FTM3-6 Y₁₀₆A, or FTM3-6 W₉₈Y₁₀₆AA. After 24 h, GST, GTM1-2ΔC, or GTM1-2ΔC A₃₈ALA₄₁ was adsorbed to glutathione-conjugated Sepharose beads. The proteins pulled down by the beads were eluted with sodium dodecyl sulfate gel loading buffer (GST PD) and were identified by polyacrylamide gel electrophoresis and immune blotted for the LMP1 C terminus (CT) (top) or GST (bottom). Three percent of the WCE was also immune blotted for the LMP1 C terminus (middle). The data were analyzed by using Molecular Imaging software (Kodak). The band intensity for wild-type intermolecular association with GTM1-2ΔC was normalized as 100%, and relative intermolecular association percentages were computed accordingly. The data shown are representative of three independent experiments.