FIG. 1.

SPR analysis of envelope glycoprotein E1-heparin binding. (A) Heparin-BSA or BSA was covalently immobilized onto the surface of a biosensor chip as described recently (2). Subsequently, different concentrations of recombinant highly purified envelope glycoprotein E1 were injected onto the biosensor surface. The biosensor chip response is indicated on the y axis (measured in RU) as a function of time (x axis) at 25°C. The sensorgram shows the difference in the BSA-heparin coated chip response compared to the BSA-coated control chip response following E1 injection. (B) Side-by-side analysis of E1 and E2 (both at 100 nM).