FIG. 3.

(A) Transfection of repair HearNPV bacmid. (B) Infection assay for viral propagation. (C) Western blot analysis of repair HearNPV BVs. HzAM1 cells were transfected with repaired HearNPV bacmids (positive control and Tr-6aa, Tr-16aa, Tr-19aa, Tr-29aa, Tr-40aa, and Tr-49aa mutants). GFP expression in each transfection was examined 5 days later (A). Supernatants were collected at 7 days p.t. After being clarified, the supernatants were used to infect new HzAM1 cells. GFP expression, indicating infectivity, was examined 3 days after infection (B). Western blot analysis was performed on purified wild-type HearNPV BVs (WT) and f-repaired HearNPV BVs (positive control and Tr-6aa and Tr-16aa mutants), using anti-F₁ and anti-F₂ under either reducing or nonreducing conditions.