FIG. 7.

Detection of ie-1 gene early transcripts upon nonproductive infection of pseudotyped AcMNPVs in HzAM1 cells. HzAM1 cells were infected with gp64-null AcMNPVs pseudotyped with Tr-6aa, Tr-16aa, Tr-19aa, Tr-29aa, Tr-40aa, and Tr-49aa and with wild-type AcMNPV. At 12 h p.i., total RNA from infected cells of each infection was isolated. 3′ RACE analysis of the AcMNPV ie-1 gene was performed. PCR-amplified products were analyzed in a 1% agarose gel. Total RNA samples from each treatment were analyzed as template controls by reverse transcription.