FIG. 4.

Subcellular localization of wt NS5B and of its variants in Huh-7 cells. (A) Amino acid sequences of the wt and mutant NS5B C-terminal transmembrane domains. The foreign amino acid sequences derived from poliovirus 3A are underlined. The positively charged amino acids located at both ends of the hydrophobic domains of HCV NS5B and poliovirus 3A are marked in bold. NS5B(CΔ21) represents a NS5B protein lacking the C-terminal 21 amino acids. (B) The wt and mutant NS5B proteins were transiently expressed in Huh-7 cells and detected by an indirect immunofluorescence assay with monoclonal antibody 5B-12B7 as described in Materials and Methods.