TABLE 2.
Dissociation constants for the interaction of N protein trimers, from Sin Nombre, Andes, Puumala, Prospect Hill, and Seoul viruses with wild-type S segment panhandles from different viruses in binding buffer containing 160 mM NaCl at 25°Ca
| Panhandle |
KD (nM) for interaction with indicated N protein
|
||||
|---|---|---|---|---|---|
| SNV | Andes virus | Puumala virus | PHV | Seoul virus | |
| Andes virus | 27 ± 3 | 17 ± 2 | 18 ± 2 | 15 ± 1 | 15 ± 1 |
| PHV | 114 ± 3 | 15 ± 2 | 21 ± 2 | 13 ± 1 | 13 ± 1 |
| Puumala virus | 103 ± 4 | 18 ± 1 | 17 ± 1 | 14 ± 1 | 31 ± 2 |
| Seoul virus | 97 ± 4 | 21 ± 2 | 18 ± 1 | 17 ± 1 | 10 ± 1 |
| SNV | 29 ± 2 | 19 ± 2 | 19 ± 1 | 19 ± 1 | 11 ± 1 |
| Bunyamwera virus | 228 ± 6 | 101 ± 5 | 113 ± 5 | 109 ± 4 | 103 ± 3 |
| Tomato spotted wilt virus | 185 ± 5 | 17 ± 4 | 115 ± 6 | 111 ± 3 | 97 ± 4 |
| Rift Valley fever virus | 201 ± 5 | 125 ± 4 | 119 ± 4 | 103 ± 4 | 102 ± 2 |
| CCHFV | 229 ± 7 | 119 ± 2 | 117 ± 5 | 117 ± 4 | 91 ± 3 |
a
PHV, Prospect Hill virus; CCHFV, Crimean Congo hemorrhagic fever virus.