TABLE 4.
Stern-Volmer quenching constants for the association of N proteins from Sin Nombre, Andes, Puumala, Prospect Hill, and Seoul viruses with wild-type S segment panhandles from different viruses in binding buffer containing 80 mM NaCl at room temperaturea
| Panhandle | Quenching constant for interaction with indicated N protein
|
||||
|---|---|---|---|---|---|
| SNV | Andes virus | Puumala virus | PHV | Seoul virus | |
| None | 6.4 ± 0.1 | 7.2 ± 0.3 | 9.7 ± 0.4 | 7.9 ± 0.1 | 6.3 ± 0.1 |
| Andes virus | 2.8 ± 0.05 | 2.4 ± 0.03 | 4.2 ± 0.1 | 5.2 ± 0.3 | 3.3 ± 0.2 |
| PHV | 5.2 ± 0.1 | 2.7 ± 0.1 | 4.8 ± 0.3 | 4.9 ± 0.2 | 3.1 ± 0.2 |
| Puumala virus | 5.4 ± 0.1 | 2.7 ± 0.02 | 3.5 ± 0.1 | 5.4 ± 0.3 | 3.6 ± 0.1 |
| Seoul virus | 4.0 ± 0.3 | 3.0 ± 0.2 | 4.5 ± 0.2 | 5.1 ± 0.2 | 3.0 ± 0.3 |
| SNV | 2.6 ± 0.06 | 2.5 ± 0.1 | 4.3 ± 0.1 | 5.3 ± 0.2 | 3.3 ± 0.3 |
| Bunyamwera virus | 5.0 ± 0.3 | 5.9 ± 0.3 | 8.1 ± 0.4 | 7.1 ± 0.2 | 5.2 ± 0.1 |
| Tomato spotted wilt virus | 5.4 ± 0.2 | 6.2 ± 0.1 | 8.0 ± 0.2 | 7.3 ± 0.1 | 5.6 ± 0.1 |
| Rift Valley fever virus | 5.0 ± 0.3 | 6.3 ± 0.2 | 8.6 ± 0.3 | 7.7 ± 0.2 | 5.3 ± 0.2 |
| CCHFV | 4.9 ± 0.2 | 6.8 ± 0.3 | 8.8 ± 0.4 | 7.2 ± 0.2 | 5.9 ± 0.1 |
a
Tryptophan fluorescence of N protein (25 nM) was quenched with acrylamide in the presence or absence panhandle RNA to generate the Stern-Volmer plots. The panhandle RNA concentration was three times the dissociation constant for the panhandles used in each experiment. PHV, Prospect Hill virus; CCHFV, Crimean Congo hemorrhagic fever virus.