TABLE 5.
Dissociation constants for purified trimeric N proteins from Andes, Puumala, Prospect Hill, Seoul, and Sin Nombre viruses with different panhandles in binding buffer at 25°C
| Proteina | Panhandle |
KD (nM) at indicated NaCl concn
|
|
|---|---|---|---|
| 80 mM | 220 mM | ||
| Andes virus | SNV S mutant 1 | 27 ± 3.1 | 31 ± 3.2 |
| Puumala virus | 25 ± 3.5 | 29 ± 2.0 | |
| PHV | 22 ± 4.1 | 27 ± 3.3 | |
| Seoul virus | 21 ± 2.0 | 27 ± 2.6 | |
| SNV | 212 ± 4.0 | ||
| Andes virus | SNV S mutant 2 | 257 ± 3.1 | |
| Puumala virus | 227 ± 5.2 | ||
| PHV | 262 ± 4.1 | ||
| Seoul virus | 229 ± 3.0 | ||
| SNV | 319 ± 4.0 | ||
| Andes virus | SNV S mutant 3 | 26 ± 2.1 | 31 ± 3.0 |
| Puumala virus | 19 ± 3.2 | 29 ± 2.2 | |
| PHV | 27 ± 4.1 | 31 ± 2.1 | |
| Seoul virus | 21 ± 1.0 | 27 ± 1.5 | |
| SNV | 22 ± 1.0 | 25 ± 2.1 | |
a
PHV, Prospect Hill virus.