FIG. 3.

UNG2 binds to the carboxyl terminus of LANA. The indicated LANA deletion mutant forms were in vitro translated with a transcription-translation kit in the presence of [³⁵S]methionine and analyzed for binding with either GST or GST-UNG2. LANA deletion mutant forms containing the carboxyl terminus (amino acids 762 to 1162, 1 to 327, 929 to 1172, and 946 to 1162) bound to GST-UNG2. Bound fractions were quantified, and the relative binding with each LANA deletion mutant form, calculated on the basis of three independent experiments, is shown as a bar graph.