FIG. 7.

KSHV virions infect UNG2-deficient (UNG^−/−) MEFs with an efficiency similar to that with which they infect wild-type (UNG^+/+) MEFs. (A) Immunolocalization of LANA in KSHV-infected MEFs. (B) Quantitation of KSHV virions obtained from infected UNG^−/− and UNG^+/+ MEFs showed an approximately 30% reduction in the number of virions obtained from equal numbers of infected MEFs. (C) Detection of RTA in KSHV-infected UNG^−/− and UNG^+/+ cells after induction with TPA and sodium butyrate. (D) LANA immunostaining in HEK293 cells infected with KSHV virions obtained from UNG^−/− and UNG^+/+ cells. Infection of HEK293 cells with the viruses obtained from MEFs was greatly reduced, with almost no LANA detection in HEK293 cells infected with virus from UNG^−/− cells, and only a few UNG^+/+ cells produced virus.