FIG. 2.

HSV-1 capsids and glycoprotein gD do not colocalize in SK-N-SH neurons. Human SK-N-SH neurons were infected with HSV-1 (F) for 18 h, fixed with 4% paraformaldehyde, and permeabilized with 0.2% Triton X-100. (A) The neurons were stained with a combination of two mouse MAbs specific for VP5 (red) and, simultaneously, with rabbit polyclonal antibodies specific for gD (green), followed by Alexa 594-conjugated goat anti-mouse IgG and Alexa 488-conjugated goat anti-rabbit IgG secondary antibodies. (B) Higher magnification of panel A showing individual VP5 (red) and gD (green) puncta. The inset in panel B represents rare examples of overlapping VP5 (red) and gD puncta (green). Single sections of 200 nm are shown. Scale bars are 4 μm in panel A and 1 μm in panel B.