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. 2006 Sep 13;80(22):11165–11177. doi: 10.1128/JVI.01107-06

FIG. 8.

FIG. 8.

HSV glycoproteins are transported normally into axons when capsids are retained in neuronal cell bodies. SK-N-SH neurons were infected with wild-type HSV-1 (KOS) (A and B) or TsProt.A (C and D) for 90 min at 37°C then shifted to 39.5°C for 16.5 h. Neurons were stained with a pool of anti-VP5 MAb (red) and rabbit polyclonal gD-specific antibodies (green) (A and C) or rabbit polyclonal gE/gI-specific antibodies (green) (B and D) followed by Alexa 594-conjugated anti-mouse IgG and Alexa 488-conjugated anti-rabbit IgG. Scale bars, 6 μm.