FIG. 1.
Map of MBP-Rep120-359. The white box (aa 1 to 390) represents MBP, the line corresponds to the linker region with the enterokinase (EK) cleavage site, and the two factor Xa cleavage sites (121/122 in Rep) are indicated. The Rep120-359 region is constituted by the oligomerization domain (aa 120 to 180, black box), the ATPase domain (aa 181 to 330, gray box), and the C-terminal region (aa 331 to 359, white box) of unknown function. The conserved Walker A (WA, aa 217 to 230), Walker B (WB, aa 257 to 263), and C (aa 300 to 305) are indicated, as is the position of the mutated K at position 227 in the P loop.