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Rep122-359 helicase activity on M13-derived substrates. The percentage of unwinding as a function of Rep122-359 concentration in picomoles per assay is shown with three different substrates (a [▪], b [▴], and c [•]), as are the corresponding gels. For a and c, lanes 1 and 2 are substrate alone nonboiled and boiled, respectively; for b, lanes 1 and 2 are boiled and native substrate, respectively. Lanes 3 to 9 show increasing concentration of Rep122-359 as indicated on the graph.
