FIG. 4.

Rep122-359 helicase activity on forked and flap substrates. A, percentage of unwinding of four different forked substrates (d[▪], e [▾], f [•], and g [▵]), with different lengths of ss tails as indicated, presented as a function of Rep122-359 concentration. The corresponding gels are displayed; for d, e, and g, lanes 1 and 2 show the substrate alone native and boiled, respectively, and for f, lanes 1 and 2 show boiled and native substrate, respectively. B, unwinding of three flap substrates h, i, and j with different length of overhang as indicated and as a function of time. Each reaction mixture contained 2 fmol of substrate and 3 pmol of Rep122-359. The percentage of unwinding is indicated at the bottom of the gel, and the time intervals are indicated above the gel.