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. 2006 Aug 30;80(22):11322–11330. doi: 10.1128/JVI.00924-06

FIG. 5.

FIG. 5.

Glycerol gradient sedimentation of MBP-Rep120-359 and Rep122-359. A, SDS-PAGE of fractions 7 to 19 (from bottom to top of the gradient) corresponding to MBP-Rep122-359 sedimentation. B, SDS-PAGE of MBP and Rep122-359 after factor Xa cleavage (fractions 7 to 23). Lanes M, molecular mass markers (same as in Fig. 2). C, ATPase activity expressed as absorbance at 650 nm for MBP-Rep122-359 (▴) and Rep122-359 (▪) for the same fractions as in A and B. β-Galactosidase (15.9 S) migrates in fraction 6 in both gradients (not shown).