FIG. 5.

ICP0 null mutants do not reactivate in vivo. Mice were infected on the cornea (strain 17syn+) or the cornea plus the snout (dl1403 and FXE) with the indicated inoculation titers. The percentages of neurons in which latency was established were determined by PCR analysis of individual neurons as described in Materials and Methods. The numbers within the bars are the numbers of neurons positive/tested. Additional animals were subjected to hyperthermic stress, and ganglia were analyzed for infectious virus at 22 h poststress. The percentages of mice positive for infectious virus and the numbers of mice positive/tested are indicated.