FIG. 3.
The extent of CA-SP1 processing does not correlate with PA-457 resistance. HeLa cells were transfected with WT pNL4-3 or derivatives containing the indicated mutations and pulse-labeled for 15 min with [35S]Met/Cys. Cells were chased for the indicated times in unlabeled medium, and cell lysates were immunoprecipitated with HIV-Ig. The extent of processing of CA-SP1 to CA was analyzed by SDS-PAGE and fluorography (A and B) followed by phosphorimager analysis to quantify the percentage of CA-SP1 relative to total CA-SP1 plus CA (C and D). Error bars indicate standard errors of the means (n = 4 [C] or 3 [D]).