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Replication of SP1-A3V at various concentrations of PA-457. Ten flasks of Jurkat T cells transfected with pNL4-3-SP1-A3V were cultured in parallel in the presence of 50 ng/ml or 1.0 μg/ml PA-457. Cells transfected with pUC19, WT pNL4-3, or pNL4-3-SP1-A3V, cultured without PA-457, served as controls. Cells were split every 2 days, and supernatants were reserved at each time point for RT analysis.
