TABLE 1.
Primers used for epitope mapping recognized by Res2-6C8 and Res2-1D8
| Primer | Sequence (5′ to 3′)a | Template of reaction |
|---|---|---|
| R635f | CAAGGATCCGAAGACCCTGATATC | pGEX-ΔNP631-739 |
| R636f | TTGGGATCCGACCCTGATATCGGTCAATC | cDNA of EBO-R |
| R637f | TTGAATGGATCCCCTGATATCGGT | pGEX-ΔNP631-739 |
| R638f | AATGAAGGATCCGATATCGGTCAA | pGEX-ΔNP631-739 |
| Mu639f | TGAAGACCCTGATTAAGGTCAATCAAAGT | pGEX-ΔNP631-739 |
| Mu639r | ACTTTGATTGACCTTAATCAGGGTCTTCA | pGEX-ΔNP631-739 |
| Mu640f | AGACCCTGATATCTAACAATCAAAGTCTA | pGEX-ΔNP631-739 |
| Mu640r | TAGACTTTGATTGTTAGATATCAGGGTCT | pGEX-ΔNP631-739 |
| Mu641f | CCCTGATATCGGTTAATCAAAGTCTATGC | pGEX-ΔNP631-739 |
| Mu641r | GCATAGACTTTGATTAACCGATATCAGGG | pGEX-ΔNP631-739 |
| Mu642f | TGATATCGGTCAATAAAAGTCTATGCAAA | pGEX-ΔNP631-739 |
| Mu642r | TTTGCATAGACTTTTATTGACCGATATCA | pGEX-ΔNP631-739 |
| Mu643f | TATCGGTCAATCATAATCTATGCAAAAAT | pGEX-ΔNP631-739 |
| Mu643r | ATTTTTGCATAGATTATGATTGACCGATA | pGEX-ΔNP631-739 |
| Mu644f | CGGTCAATCAAAGTAAATGCAAAAATTAG | pGEX-ΔNP631-739 |
| Mu644r | CTAATTTTTGCATTTACTTTGATTGACCG | pGEX-ΔNP631-739 |
a
BamHI restriction site is underlined. Introduced stop codon is in boldface.