FIG. 1.

Inhibition of viral entry by drugs that target different internalization pathways. Huh7 cells were treated with chlorpromazine (25 μM), filipin (5 μg/ml), or nystatin (12.5 μg/ml) for 1 h at 37°C. The mock treatments were water for chlorpromazine and nystatin as well as methanol for filipin. Cells were infected with HCVpp, SFVpp, VSVpp, or HTLV-1pp in the continued presence of drugs or corresponding mock treatment. Media were changed after 2 h, and luciferase activity in cellular extracts was measured 48 h postinfection. The percent entry was calculated relative to mock-treated cells, and values are means of at least three independent experiments ± standard deviations.