Skip to main content
. 2006 Sep 27;80(23):11571–11578. doi: 10.1128/JVI.01717-06

FIG. 2.

FIG. 2.

Inhibition of pseudoparticle entry by agents that target clathrin-mediated endocytosis. (A) Huh7 cells were lipofected with a nonspecific siRNA (control) or an siRNA targeting the clathrin HC (α-CHC). Seventy-two hours posttransfection, cellular extracts were analyzed for clathrin HC and β-tubulin expression by Western blotting. (B) Huh7 cells were infected with HCVpp or HTLV-1pp 72 h after transfection with siRNAs. Luciferase activity in cell lysates was measured 48 h postinfection. The percent entry was calculated relative to the nonspecific siRNA-transfected cells. Results are from at least three independent experiments ± standard deviations. (C) Huh7 cells were transduced with pQCXIP backbone vector, pQCXIP-EGFP-Eps15(D3Δ2), or pQCXIP-EGFP-Eps15(Δ95/295). Cells were infected with HCVpp, SFVpp, or HTLV-1pp 48 h posttransduction. Luciferase activity in cell lysates was measured 48 h postinfection. The percent entry was calculated relative to the backbone vector-transduced cells. Results are from at least three independent experiments ± standard deviations.