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Detection of virus-specific IgG1 and IgG2a in sera of primed mice. Young adult (8-week-old) or aged (18-month-old) mice were primed i.n. with 2 × 105 PFU of ca Sing or ca Sing-IL-2. Serum samples were obtained 24 days after immunization. Virus-specific serum IgG1 (gray bars) and IgG2a (black bars) levels were determined by ELISA and are presented as geometric mean titers.
