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Detection of virus-specific IgA in nasal and vaginal secretions of primed mice. Young adult (8-week-old) or aged (18-month-old) mice were primed i.n. with 2 × 105 PFU of ca Sing (black bars) or ca Sing-IL-2 (hatched bars). Mouse nasal (a) or vaginal (b) secretions were obtained 3 weeks after immunization. Virus-specific IgA titers were determined by ELISA of pooled mouse samples processed as described in Materials and Methods.
