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Detection of KV in the Varroa mites that parasitized the honeybees. The Varroa mites that were attached to workers were collected on 24 November, 30 November, and 3 December, and the amounts of Kakugo RNA were quantified using quantitative RT-PCR. The relative Kakugo RNA contents normalized with that of Varroa destructor actin mRNA are indicated by dots. The numbers of collected mites and KV-positive mites and the detection rates are shown below.
