TABLE 1.
PPMO sequences and target locations in CVB3 RNA
| PPMO | Sequence (5′-3′) | Target location in CVB3 RNAa |
|---|---|---|
| 1 | CAACCCACAGGCTGTTTTAA | +, 5′ terminus (1-21) |
| 2 | AAATTCTCCGCATTCGGTGCG | −, 5′ terminus |
| 3 | CGCACCGAATGCGGAGAATTT | +, 3′ terminus (7378-7398) |
| 4 | TTAAAACAGCCTGTGGGTTG | −, 3′ terminus |
| 5 | CCCATTTTGCTGTATTC | +, AUG region (730-746) |
| 6b | ATGAAACACGGACACCCAAAG | +, IRES core sequence (547-567) |
| 7 | TAAGCAGCCAGTATAGGAATA | +, IRES core sequence (570-590) |
| 8 | CAATGGGCCTGTGGGTGGG | + (23-41) |
| Cc | AGTCTCGACTTGCTACCTCA |
a
Based on the complete CVB3 genome sequence (GenBank accession no. M33854). Target locations and nucleotide positions (in parentheses) in the virus-positive (+) genomic or virus-negative (−) antigenomic strand are noted.
b
Prepared as both PMO and PPMO (see Materials and Methods).
c
C, negative control (random sequence). Prepared in four different forms: PMO-C, PPMO-C, Fl-PMO-C, and Fl-PPMO-C (see Materials and Methods).