FIG. 3.

Characterization of env gene in the provirus harbored in patient SG1. The upper part of the figure shows a schematic representation of the first and second rounds of the PCR protocols designed for the amplification of the proximal (A upper), middle (B upper), and distal (C upper) parts of the env gene. Nucleotide numbering refers to HXB2 sequence positions. The lower part of the figure shows the gel patterns of the nested-PCR products for the proximal (A lower) and distal (C lower) tracts of env. The lengths of the polymorphisms of the clones derived from the PCR product designed for the middle part of env are shown in panel B lower. cl-1, clone no. 1; cl-3, clone no. 3; etc.