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Alignment of the entire Env protein. Shown are sequences of proximal and distal parts of Env and of the subclones (cl-1, cl-3, cl-4, cl-5, cl-6, and cl-7) derived from a PCR performed for the middle part of Env. The changed amino acids are shown in bold characters. Dashes indicate the deleted amino acids. The start and end of the V1, V2, V3, V4, and V5 domains are indicated by vertical lines. Two oblique lines indicate the gp120-gp41 proteolytic domain.
