FIG. 1.

Establishment and validation of the NEC PCA. (A) Simplified scheme of a PCA. The N- and C-terminal parts of a reporter enzyme (white), here the TEM-1 β-lactamase (Bla), are fused to two interacting proteins (shapes labeled A and B). If proteins A and B interact, the proximity of the enzyme fragments BlaN and BlaC allows the folding of the active enzyme (star). (B) Schematic representation of the used HA (H)-tagged constructs. The N-terminal part of Bla, representing residues 24 to 194 (N), and the C-terminal part, representing the Bla residues 196 to 286 (C), were fused to the MCMV proteins M50 (WT or mutant) and M53, linked by a glycine/serine spacer (S).