TABLE 1.
Mimivirus proteins identified in this study
| Protein name and category | ORFa | 2D spot no.b |
|---|---|---|
| DNA topology and repair | ||
| Topoisomerase IB (pox-like) | MIMI_R194 | NP |
| Topoisomerase IA (bacterial type—untwisting) | MIMI_L221 | NP |
| DNA polymerase family X | MIMI_L318 | NP |
| Regulator of chromosome condensation RCC1 | MIMI_R345 | 1, 2, 3, 4, 5 |
| DNA UV damage repair endonuclease | MIMI_L687 | NP |
| Transcription | ||
| DNA-directed RNA polymerase subunit 5 | MIMI_L235 | 93 |
| DNA-directed RNA polymerase subunit 2 | MIMI_L244 | NP |
| VV D6R-like helicase | MIMI_R350 | NP |
| DNA-directed RNA Pol2 G subunit | MIMI_L376 | 55 |
| D6R-like putative early transcription factor | MIMI_L377 | NP |
| African swine fever virus (ASFV) NP868R-like mRNA capping enzyme | MIMI_R382 | NP |
| DNA-directed RNA polymerase subunit L | MIMI_R470 | 37 |
| DNA-directed RNA polymerase subunit 1 | MIMI_R501 | NP |
| VV I8-like helicase | MIMI_L540 | NP |
| NPH-1-like transcription termination factor | MIMI_L538 | NP |
| TFIIB-like transcription initiation factor | MIMI_L544 | NP |
| NPH-1-like transcription termination factor | MIMI_R563 | NP |
| Oxidative pathways | ||
| Choline dehydrogenase | MIMI_R135 | 69, 70, 71, 72 |
| Amine oxidoreductase | MIMI_R188 | 10 |
| Glutaredoxin (ESV128 type) | MIMI_R195 | NP |
| Thioredoxine domain | MIMI_R362 | 96 |
| Thioredoxine domain | MIMI_R443 | 67 |
| Zn-dependent alcohol dehydrogenase | MIMI_L498 | NP |
| Putative oxidoreductase (C terminus) | MIMI_L893 | NP |
| Putative oxidoreductase (N terminus) | MIMI_L894 | NP |
| Thiol oxidoreductase E10R (C-C bonding) | MIMI_R596 | 87 |
| Protein/lipid modification | ||
| Ubiquitin-specific protease | MIMI_L293 | NP |
| Serine/threonine protein phosphatase | MIMI_R307 | NP |
| Phosphoesterase | MIMI_R398 | NP |
| F10L-like S/T protein kinase | MIMI_R400 | NP |
| Putative S/T protein kinase | MIMI_L516 | NP |
| Putative triacylglycerol lipase | MIMI_R526 | 45, 46 |
| Prolyl 4-hydroxylase | MIMI_L593 | NP |
| Particle structure | ||
| A16L-like virion-associated membrane protein | MIMI_L65 | NP |
| Major core protein | MIMI_L410 | 11, 12 |
| Capsid protein D13L | MIMI_L425 | 6, 7, 8, 9 |
| Putative spore coat assembly factor | MIMI_L454 | NP |
| Lipocalin-like outer membrane lipoprotein | MIMI_R877 | NP |
| Other | ||
| Cytoskeletal protein | MIMI_L294 | 21, 22 |
| RNA methyl transferase | MIMI_R383 | NP |
| tRNA (uracil-5-)methyltransferase | MIMI_R407* | 88, 89 |
| Putative exoribonuclease | MIMI_R528 | NP |
| Cytochrome P450 | MIMI_L532* | 75 |
| Putative elongation factor EF-Tu homolog | MIMI_R553 | 73, 74 |
| Proline-rich protein | MIMI_R610 | 59, 60 |
| Mannose-6-phosphate isomerase | MIMI_L612 | 68 |
| Phosphatidyl-ethanolamine-binding protein | MIMI_R644 | NP |
| Chemotaxis | MIMI_R706 | NP |
| Chemotaxis | MIMI_R721 | 44 |
| ORFan | MIMI_L48* | 65, 66 |
| ORFan | MIMI_L208 | NP |
| Unknown (WD repeat) | MIMI_L264 | NP |
| ORFan | MIMI_L274 | NP |
| Chilo iridescent virus 380R-like anonymous domain | MIMI_R301 | NP |
| ORFan | MIMI_L309 | NP |
| ORFan | MIMI_L330 | 83 |
| ORFan | MIMI_L352 | NP |
| ORFan | MIMI_L389 | NP |
| ORFan | MIMI_L399 | NP |
| Unknown (PBCV-1 A488R) | MIMI_L417 | NP |
| ORFan | MIMI_L442 | 23, 24, 25, 26, 30, 31, 32, 33, 34, 35, 36 |
| ORFan | MIMI_L452 | NP |
| Unknown (ankyrin repeat) | MIMI_L484 | NP |
| ORFan (biotinyl carrier) | MIMI_L485* | 95 |
| ORFan | MIMI_L488 | NP |
| ORFan (coenzyme A binding) | MIMI_L492 | NP |
| Unknown | MIMI_L515 | NP |
| ORFan (nuclease) | MIMI_L533 | NP |
| ORFan (leucine zipper) | MIMI_L550 | 97 |
| Unknown | MIMI_L567 | 78, 79, 80, 81, 82 |
| ORFan | MIMI_L585 | NP |
| ORFan | MIMI_L591 | 77 |
| ORFan (leucine zipper) | MIMI_L647 | 94 |
| ORFan | MIMI_L688 | 52, 53 |
| Unknown (WD repeat) | MIMI_L690 | 27, 28, 29 |
| Unknown (COG 3979) | MIMI_L701 | NP |
| ORFan (fusion protein [Nipah virus]) | MIMI_L724 | 85, 86 |
| ORFan | MIMI_L725 | 47, 48, 49, 50, 51 |
| ORFan | MIMI_L778 | NP |
| Unknown | MIMI_L829 | 13, 14, 15, 16, 17, 18, 54, 62 |
| ORFan | MIMI_L851 | NP |
| ORFan (aromatic amino acid hydrolase) | MIMI_L872 | 43 |
| ORFan | MIMI_L899 | NP |
| ORFan | MIMI_R160 | NP |
| Unknown | MIMI_R161 | NP |
| Unknown | MIMI_R253 | 38, 39, 40, 76 |
| ORFan | MIMI_R326 | NP |
| Unknown | MIMI_R327 | NP |
| Unknown (ASFV C475L) | MIMI_R341 | 41 |
| ORFan | MIMI_R347 | NP |
| Unknown (ASFV S273R) | MIMI_R355 | NP |
| ORFan (apolipophorin III) | MIMI_R387 | 42 |
| Unknown | MIMI_R402 | 19 |
| ORFan (kinase) | MIMI_R403 | NP |
| ORFan | MIMI_R457 | NP |
| ORFan | MIMI_R459 | 91, 92 |
| ORFan | MIMI_R463 | NP |
| Unknown | MIMI_R472 | NP |
| Unknown | MIMI_R486 | NP |
| Unknown (PBCV-1 A352L) | MIMI_R489 | 56, 57, 58 |
| ORFan | MIMI_R557 | NP |
| ORFan (acetyltransferase) | MIMI_R584 | 63, 64, 84 |
| ORFan | MIMI_R646 | NP |
| Unknown (WD repeat) | MIMI_R648 | 20 |
| ORFan | MIMI_R653 | 61 |
| ORFan | MIMI_R658 | NP |
| ORFan | MIMI_R679 | NP |
| ORFan | MIMI_R691 | NP |
| ORFan | MIMI_R692 | 90 |
| ORFan | MIMI_R695 | NP |
| ORFan | MIMI_R705 | NP |
| ORFan (alpha-synuclein) | MIMI_R710 | NP |
| Unknown | MIMI_R722 | NP |
| ORFan | MIMI_R727 | NP |
a
*, ORF products identified solely through 2D MS.
b
NP, proteins not positioned on 2D gels.