TABLE 1.
Human heavy and light chain primers
| Primer | Sequence |
|---|---|
| Heavy chain, variable, 5′ specific | |
| VH1a | 5′-CAG GTG CAG CTC GAG CAG TCT GGG-3 (24-mer) |
| VH1f | 5′-CAG GTG CAG CTG CTC GAG TCT GGG-3′ (24-mer) |
| VH2f | 5′-CAG GTG CAG CTA CTC GAG TCG GG-3′ (23-mer) |
| VH3a | 5′-GAG GTG CAG CTC GAG GAG TCT GGG-3′ (24-mer) |
| VH3f | 5′-GAG GTG CAG CTG CTC GAG TCT GGG-3′ (24-mer) |
| VH4f | 5′-CAG GTG CAG CTG CTC GAG TCG GG-3′ (23-mer) |
| VH6a | 5′-CAG GTA CAG CTC GAG CAG TCA GG-3′ (23-mer) |
| Light chain, variable, 5′ specific | |
| κ | |
| VK1a | 5′-GAC ATC GAG CTC ACC CAG TCT CCA-3′ (24-mer) |
| VK1s | 5′-GAC ATC GAG CTC ACC CAG TCT CC-3′ (23-mer) |
| VK2a | 5′-GAT ATT GAG CTC ACT CAG TCT CCA-3′ (24-mer) |
| VK3a | 5′-GAAATTGAGCTCACGCAGTCTCCA-3′ (24-mer) |
| VK3b* | 5′-GAAATTGAGCTCAC(G/A)CAG TCTCCA-3′ (24-mer) |
| λ | |
| VL1 | 5′-AAT TTT GAG CTC ACT CAG CCC CAC-3′(24-mer) |
| VL2 | 5′-TCT GCC GAG CTC CAG CCT GCC TCC GTG-3′ (27-mer) |
| VL3 | 5′-TCT GTG GAG CTC CAG CCG CCC TCA GTG-3′ (27-mer) |
| VL3′ | 5′-TCCTATGAGCTCACTCAGCCACCC-3′ (24-mer) |
| VL4 | 5′-TCTGAAGAGCTCCAGGACCCTGTTGTG TCTGTG-3′ |
| VL5 | 5′-CAG TCT GAG CTC ACG CAG CCG CCC-3′ (24-mer) |
| VL6 | 5′-CAG ACT GAG CTC ACT CAG GAG CCC-3′ (24-mer) |
| VL7 | 5′-CAG GTT GAG CTC ACT CAA CCG CCC-3′ (24-mer) |
| Heavy chain, constant, 3′ specific | |
| CG1z | 5′-GCA TGT ACT AGT TTT GTC ACA AGA TTT GGG-3′ (30-mer) |
| Light chain, constant, 3′ specific | |
| κ | |
| CK1d | 5′-GCG CCG TCT AGA ATT AAC ACT CTC CCC TGT TGA AGC TCT TTG TGACGG GCG AAC TCA G-3′ (57-mer) |
| λ | |
| CL2d | 5′-AGA GAG AGA GAG AGA GAG AGC GCC GTC TAG AAT TAT GAA CAT TCT GTA GG-3′ (50-mer) |